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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants were claimed but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide generally incorporates the epitope recognized from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Target Information Cyclin E belongs to the really conserved cyclin family, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins function as get more info regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation styles which add to your temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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